CALM: Equipment

CALM
Equipment

Instruments

The Center for Advanced Light Microscopy (CALM) provides infrastructure and guidance enabling scientists to perform fluoreszence-based techniques, such as live imaging, confocal microscopy, super-resolution microscopy as well as time-resolved fluorescence (FRET, FLIM, fluorescence anisotropy, fluorescence correlation spectroscopy).

Confocal Systems

Leica TCS SP8 X WLL2 upright Hyvolution 2

Room 122, Building 4218, Emil-Ramann-Str. 4, WZW

Microscope Stand: DM6 CS, upright, motorized

Scanner: TCS confocal point scanner with AOBS and AOBM, galvo/resonance-hybrid

Objectives: HCPL APO 63x/NA 1.20/WD 0.30, Water CORR CS2; HCPL APO 63x/NA 1.30/WD 0.30, Glycerol CORR CS2; HCPL APO 40x/NA 1.10/WD 0.65, Water IMM CORR CS2; HCPL APO 20x/NA 0.75/WD 0.67, IMM CORR CS2; HCPL FLUOTAR 10x/NA 0.30/WD 11.0.

Lasers:

405 nm, 50 mW (diode); multi-argon (458 nm, 476 nm, 488 nm, 496 nm, 514 nm), 65 mW.

White-light laser (WLL): eight freely adjustable excitation wavelengths in 1 nm steps (470 - 670 nm)

Detection:

2x HyD GaAsP; 2x PMT

Software:

LAS X SP8 Control Software/Dye Finder/Live Data Mode/MicroLab; LAS X HyVolution 2

Olympus FV 3000 inverse, with Picoquant FLIM and FCS upgrade kit (time-resolved fluorescence)

Room 125, Building 4218, Emil-Ramann-Str. 4, WZW

Olympus FV 3000:

Microscope Stand: IX83, inverse, motorized

Scanner: confocal point scanner, galvo/resonance-hybrid

Objectives: UPLSAPO60XS2 60x/NA 1.3/WD 0.3, silicon oil immersion; UPLSAPO60XW 60x/NA 1.2/WD 0.28, water immersion; UPLSAPO20X 20x/NA 0.75/WD 0.6; UPLSAPO10X2 10x/NA 0.4/WD 3.1.

Lasers (all diode):

405 nm, 50 mW; 445 nm, 75 mW; 488 nm, 20 mW; 514 nm, 40 mW; 561 nm, 50 mW; 640 nm, 40 mW.

Detection:

Olympus four-channel TruSpectral detection system (2x GaAsP, 2x PMT)

Software:

Olympus FV 3000 Imaging Software, CellSens Desktop Version 1

PicoQuant advanced FCS/FLIM-FRET/rapidFLIM upgrade kit:

Laser driver:

PDL 828 SEPIA II

Pulsed lasers (diode):

440 nm (LDH-D-C-440), 485 nm (LDH-D-C-485), 560 nm (LDH-D-TA-560)

Time-correlated single photon counting (TCSPC) and event timing:

TCSPC Modul TimeHarp 260 PICO Dual

TCSPC Modul TimeHarp 260 NANO Dual

Photon counting detectors:

2x PMA Hybrid 40

Software:

PicoQuant SymPhoTime 64

Olympus FV1000 CLSM Inverse

Room 122, Building 4218, Emil-Ramann-Str. 4, WZW

Our routine workhorse.

Olympus FV 1000:

Microscope Stand: IX83, inverse, motorized

Scanner: confocal point scanner

Objectives: Air: UPLAN FLN10X (10x/0.30/WD 10), UPLAN FLN20X (20x/0.50, WD 2.1); Water: PLANAPO 40XW LSM (40x/0.90), UPLANSAPO 60XW (60x/1.2/WD 0.28), LUMPLANFL 40XW (40x/0.80/WD 3.3), LUMFL 60XW (60x/1.10/WD 1.5); Oil: UAPO/340 (40x/1.35/WD 0.1), UPLANSAPO 60X (60x/1.35/WD 0.15), UPLANSAPO 100XO (100x/1.40/WD 0.13)

Lasers:

405 nm (diode), 458/488/515 nm (multi-line Argon), 561 nm (diode); 632 nm (He-Ne).

Detection:

2x GaAsP, 3x PMT

Software:

Olympus FluoView software

FLUORESCENT MICROSCOPES

Olympus BX61 Upright

Room 125, Building 4218, Emil-Ramann-Str. 4, WZW

Olympus BX61:

Microscope Stand: BX61, upright

Objectives: Air: UPLAN FLN4X (4x/0.13/WD 17), UPLAN FLN 10X (10x/0.30/ Ph1/WD 10), UPLAN FLN 20X (20x/0.50/ Ph1/WD 2.1), UPLAN SAPO 40X (40x/0.90/WD 0.18); Oil: PLANAPO 60X (60x/1.42/WD 0.15), UPLAN FLN 100X (100x/1.30/Ph3/WD 0.2).

Specifications: The microscope is equipped with a conventional light unit (for bright field, dark field, DIC, etc.) and an Hg-Lamp for conventional fluorescence. This includes filters for GFP, YFP, RFP etc. The system is largely motorized, e. g. the different filters are coupled into the light path via the PC/program.

The instrument is also equipped with an OLYMPUS OPTIGRID system, which produces optical sections with near-confocal quality.