The Center for Advanced Light Microscopy (CALM) of imaging@TUM at the TUM School of Life Sciences at the Weihenstephan campus enables scientists to perform cutting edge light-based microscopy and microspectrometry. Fluorescence-based techniques, such as live imaging using confocal microscopy, super-resolution microscopy, as well as time-resolved fluorescence (FRET-FLIM, fluorescence anisotropy (FA), fluorescence correlation spectroscopy (FCS)) allow the in vivo study of cellular processes with unprecedented molecular detail.